Wentworth
Institute of Technology
Introduction to experimental biophysics - a laboratory guide /
Easily Get Started with Biological Experiments Tested in a pedagogical setting, the experiments follow a logical progression beginning with a DNA construct. The book starts with the basics of molecular cloning: amplifying and purifying plasmid, plasmid mapping, and using restriction enzymes. Later e...
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| Main Author: | |
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| Corporate Author: | |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Boca Raton, FL :
CRC Press, an imprint of Taylor and Francis,
2015.
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| Edition: | First edition. |
| Subjects: | |
| Online Access: |
Full text (WIT users only) |
MARC
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| 245 | 1 | 0 | |a Introduction to experimental biophysics - a laboratory guide / |c by Jay L. Nadeau. |
| 250 | |a First edition. | ||
| 264 | 1 | |a Boca Raton, FL : |b CRC Press, an imprint of Taylor and Francis, |c 2015. | |
| 300 | |a 1 online resource (197 pages) : |b 15 illustrations | ||
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| 505 | 0 | |a chapter 1 Transformation of Competent Bacteria with a DNA Plasmid / -- chapter 2 Isolation of Plasmids by Miniprep and Restriction Enzyme Analysis / -- chapter 3 Designing a New Construct Using Restriction Enzymes (Computer Lab) / -- chapter 4 Restriction Enzymes / -- chapter 5 Ligation / -- chapter 6 Screening a Ligation and Choosing Recombinants / -- chapter 7 Use of Software to Design Primers for Cloning Using the Polymerase Chain Reaction (Computer Lab) / -- chapter 8 Running the Polymerase Chain Reaction / -- chapter 9 Protein Expression and Purification / -- chapter 10 Characterization of Purified Protein / -- chapter 11 Protein Crystallization / -- chapter 12 Transfecting Mammalian Cells with Custom- Designed Constructs / -- chapter 13 Screening Fluorescent Reporter Gene Expression in Mammalian Cells / -- chapter 14 Synthesis of Quantum Dots / -- chapter 15 Synthesis of Gold Nanoparticles / -- chapter 16 Electrophysiology: Lipid Bilayer Recording / -- chapter 17 Electrophysiology Alternative: Introduction to Lipid Vesicles. | |
| 520 | 3 | |a Easily Get Started with Biological Experiments Tested in a pedagogical setting, the experiments follow a logical progression beginning with a DNA construct. The book starts with the basics of molecular cloning: amplifying and purifying plasmid, plasmid mapping, and using restriction enzymes. Later experiments deal with more advanced, emerging techniques, such as the synthesis and characterization of quantum dots and gold nanoparticles, protein crystallization, and spectroscopic techniques. | |
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| 650 | 0 | |a Biophysics. |0 sh 85014253 | |
| 650 | 0 | |a Molecular biology |x Methods |x Laboratory manuals. |0 sh 85086577 | |
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